Part:BBa_K1363201:Design
Anti-LPS factor(LALF) regulated by IPTG
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1208
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2085
Design Notes
This part had not been test yet.What we tested is that the lactose operon. We designed the part based on K541545. We found that R0010 might keep expressing genes downstream without regulation.Reason may be that Lacl protein which plays an important role in the lactose regulation process expressed by E.coli is limited. So we inserted parts which can expresses Lacl continuously in E.coli. into the origin one, so that we could strengthen the regulation by lactose. Since we had got a wonderful gene switch controlled by lactose, we put a protein generator generating signal peptides and Limulus anti-LPS factors(LALF) downstream the switch. The signal peptides helped the LALF binding to E.coli.'s LPS layer,so that the growth of E.coil. would be limited. In general, we construct our parts to realize the idea of starting the suicide process of E.coil. by adding lactose(or IPTG) into the culture medium.
Source
The part was composed by parts provided by IGEM 2013 plants.